KMID : 1141020140140020059
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Journal of Applied Oriental Medicine 2014 Volume.14 No. 2 p.59 ~ p.67
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Hair growth promoting effect of mixed medicinal herbal extract in an C57BL/6 alopecia model
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Hwang Mee-Yul
Do Eun-Ju Jung Geum-Hee Kim Mi-Ryeo
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Abstract
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Objective£ºThis study was performed to examine the effect of mixed medicinal herbal extract including Lespedeza bicolor Turcz. and 18 others (CS-10-H) on hair growth in an alopecia model of C57BL/6 mice.
Methods£ºMale mice were divided into 3 experimental groups for treatment of CS-10-H or 5% minoxidil or vehicle solution, respectively. Dorsal skin of animals were shaved and topically applied with an amount of 200 ¥ìL of test materials once a day for 3 weeks. The apparent hair growth was monitored at 5, 10, 15 and 21 days after topical application. The changes of hair density and hair thickness were evaluated by phototrichogram using folliscope. Protein expression levels of hair growth-related factors were measured by western blotting.
Results£ºApplication of minoxidil or CS-10-H stimulated the hair growth compared to vehicle treatment. Hair density of minoxidil and CS-10-H group was increased about 206% and 208% on 14 day compared to that of vehicle group, respectively. Hair thickness of minoxidil and CS-10-H group was also increased about 220 % and 200% on 14 day compared to that of vehicle group. The protein expression of hair growth factors, IGF-1 and VEGF was significantly up-regulated in CS-10-H and minoxidil group than in vehicle treated group.
Conclusion£ºThese data suggest that CS-10-H shows potential usefulness as ingredients of hair tonic and hair-restorer for development of cosmetics and quasi-drugs, as result of its potent stimulating activity on hair growth in C57BL/6 alopecia model.
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KEYWORD
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hair growth, Lespedeza bicolor, alopecia, IGF-1, VEGF
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